A Requirement for IL-2/IL-2 Receptor Signaling in Intrathymic Negative Selection

Signaling in Intrathymic Negative Selection A Requirement for IL-2/IL-2 Receptor

No requirement for TRAIL in intrathymic negative selection.

The contribution of the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) pathway to intrathymic negative selection is a controversial subject with two studies suggesting a key role for TRAIL, while others demonstrated normal negative selection, in TRAIL- and TRAIL receptor-deficient mice. The basis of these discrepancies is unclear and may in part reflect differences in the negat...

Role of IL-12 in intrathymic negative selection.

Cytokines are central regulatory elements in peripheral lymphocyte differentiation, but their role in T cell ontogeny is poorly defined. In the present study, we evaluated the role of IL-12 in thymocyte selection more directly by determining its role in two models of in vivo negative selection. In initial studies we demonstrated that abundant intrathymic IL-12 synthesis occurs during OVA peptid...

Intrathymic Antigen Recognition and Il - 2 Receptors

The thymus plays a vital role in the ontogeny of T lymphocytes. Within this organ, T cell precursors acquire the phenotypic markers and the functional reactivities characteristic of mature T cells (1). In addi t ion, nonlymphoid components of the thymus influence the antigen recognition repertoire of developing pre-T cells such that the mature T cells that derive from them recognize foreign ant...

Requirement of a conservative cytotoxicity assay selection in cellular experiments

Assessment of the cellular effects of natural and/or synthetic products is one of the major goals in biological experiments. This assessment may be conducted at different levels; cellular viability, cellular biological activity, and cellular structure and morphology. Different assays are available for any of above purposes. It is becoming of a routine exercise to apply any of these assays, and ...